Monday, August 15, 2011

Chromatography

(Same lesson as flame test lesson)
group member: yixin

Our chromatography was quite failed. But oh well.

Step 1. Cut up portions of the cheque amount in $. In this case, '9', '0', '0', '0', '0'.
Step 2. Drop a few drops of ethanol into 5 microfuge tubes and label them '1' to '5'.
Step 3. Place the cut up numbers into the tubes, making sure the digits 1 to 5 correspond with the ordinal number of the digits on the cheque.
Step 4. Draw a line 5cm from the bottom of the 6 strips of chromatography paper.
Step 5. Use a capillary tube to drop one drop of the ink from each of the microfuge tubes onto the line on the chromatography paper, and label the chromatography paper accordingly.
Step 6. Dot one strip of chromatography paper with Marker 1/2 (depending on the group)
Step 7. Pour water into the boiling tubes and clip the chromatography paper to the tubes such that the water is just below the drop of ink/the pencil line (oh dear I think I forgot my chem...)
Step 8. After a period of time take note of the solvent front and calculate the retention factor (Rf value)

I think we didn't exactly plan our methodology properly (The above is sorta edited), hence the marks weren't very clear and we didn't manage to solve the mystery. :(

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